Analyte quantification
Analyte quantification - contextQuantification of analyte
Quantification and standards
External standard (ES) calibration advantage - disadvantage 
Signal drift and ES calib. Matrix effects and ES calib. Analyte loss and ES calib. Internal standard (IS) calibration advantage - disadvantage Signal drift and IS calib. Matrix effects and IS calib. Analyte loss and IS calib. 
Calibration by standard additionWorking range of the detectorNoise of the detectorLimit of detection and quantitationAnalyte recoveryEvaluation of an analytical method
Spike-recovery experiments using SS
End of lessonSpike-recovery experiments using surrogate standards
The animation below is a repetition on the use of a surrogate standard to calculate the analyte recovery of a given analytical method.
Whereas in the previous spike schemes, only one analyte mass (i.e., 30) was spiked, this amount will be varied in the following. This allows construction of 'calibration curves'. Of course, as in the case of IS calibration, the spiked mass of surrogate standards is always the same.
