Analyte quantification
Analyte quantification - contextQuantification of analyte
Quantification and standards
External standard (ES) calibration advantage - disadvantage 
Signal drift and ES calib. Matrix effects and ES calib. Analyte loss and ES calib. Internal standard (IS) calibration advantage - disadvantage Signal drift and IS calib. Matrix effects and IS calib. 
Analyte loss and IS calib. 
Calibration by standard additionWorking range of the detectorNoise of the detectorLimit of detection and quantitationAnalyte recoveryEvaluation of an analytical methodSpike-recovery experiments using SS
End of lessonAnalyte loss and IS calibration
example: loss of analyte during solid phase extraction
Analyte and surrogate standard have about the same fractional losses, such that their area ratios remain constant.
Hence, in contrast to ES calibration, IS calibration accounts for losses during sample preparation.
