Analyte quantification
Analyte quantification - contextQuantification of analyte
Quantification and standards
External standard (ES) calibration advantage - disadvantage 
Signal drift and ES calib. Matrix effects and ES calib. Analyte loss and ES calib. Internal standard (IS) calibration advantage - disadvantage Signal drift and IS calib. Matrix effects and IS calib. Analyte loss and IS calib. 
Calibration by standard additionWorking range of the detectorNoise of the detectorLimit of detection and quantitation
Analyte recoveryEvaluation of an analytical methodSpike-recovery experiments using SS
End of lessonAnalyte recovery
... the ratio of analyte mass in the sample prior to its preparation to the quantified analyte mass following the preparation, separation, and detection steps
|
|
| recovery | meaning and implication |
| 100% | no analyte is lost; ideal situation |
| >80% | analyte is lost, acceptable if reproducible |
| <70% | significant analyte loss, method needs to be modified to increase analyte mass recovery |
