Analyte quantification
Analyte quantification - contextQuantification of analyte
Quantification and standards
External standard (ES) calibration advantage - disadvantage 
Signal drift and ES calib. Matrix effects and ES calib. Analyte loss and ES calib. Internal standard (IS) calibration advantage - disadvantage Signal drift and IS calib. Matrix effects and IS calib. Analyte loss and IS calib. 
Calibration by standard additionWorking range of the detectorNoise of the detector
Limit of detection and quantitationAnalyte recoveryEvaluation of an analytical methodSpike-recovery experiments using SS
End of lessonLimit of detection and quantitation
limit of detection (LOD):lowest possible analyte concentration that can be reliably detected using a specific analytical method. |
limit of quantitation (LOQ):lowest possible analyte concentration that can be reliably quantified using a specific analytical method. |
The lower LOD and LOQ, the better the analytical method
The analytical method can be improved by ...
(i) reducing the background noise (i.e., reducing)
... and/or ...
(ii) increasing the slope* RF of the regression curve
* the slope is also referred to as the sensitivity of the method; do not confuse sensitivity with the limit of detection LOD
Can you think of another criterion that describes the performance of an analytical method?
