Analyte quantification

Analyte quantification - context
Quantification of analyte
Quantification and standards
     External standard (ES) calibration
           advantage - disadvantage
                 Signal drift and ES calib.
                 Matrix effects and ES calib.
                 Analyte loss and ES calib.
     Internal standard (IS) calibration
           advantage - disadvantage
                 Signal drift and IS calib.
                 Matrix effects and IS calib.
                 Analyte loss and IS calib.
     Calibration by standard addition
Working range of the detector
Noise of the detector
Limit of detection and quantitation
Analyte recovery
Evaluation of an analytical method
Spike-recovery experiments using SS
End of lesson
  

Internal standard (IS) calibration

advantage:
IS calibration accounts for potential drift of the detector, matrix effects, and analyte loss

disadvantage:
more labor intensive than ES calibration

 

Can you explain how IS calibration accounts for these effects?

(hint: how does IS calibration differ from ES calibration?)

(i) signal drift

     
  click here for explanations    
     

(ii) matrix effects

(iii) analyte loss