Analyte quantification

Analyte quantification - context
Quantification of analyte
Quantification and standards
     External standard (ES) calibration
           advantage - disadvantage
                 Signal drift and ES calib.
                 Matrix effects and ES calib.
                 Analyte loss and ES calib.
     Internal standard (IS) calibration
           advantage - disadvantage
                 Signal drift and IS calib.
                 Matrix effects and IS calib.
                 Analyte loss and IS calib.
     Calibration by standard addition
Working range of the detector
Noise of the detector
Limit of detection and quantitation
Analyte recovery
Evaluation of an analytical method
Spike-recovery experiments using SS
End of lesson

Analyte loss and ES calibration

 

example: loss of analyte during solid phase extraction

external standards are not carried through the sample preparation steps

hence, if analyte is lost in this step, quantification by ES calibration results in systematically lower than actual sample concentrations