Analyte quantification
Analyte quantification - contextQuantification of analyte
Quantification and standards
External standard (ES) calibration advantage - disadvantage 
Signal drift and ES calib. Matrix effects and ES calib. Analyte loss and ES calib. Internal standard (IS) calibration advantage - disadvantage 
Signal drift and IS calib. Matrix effects and IS calib. Analyte loss and IS calib. 
Calibration by standard additionWorking range of the detectorNoise of the detectorLimit of detection and quantitationAnalyte recoveryEvaluation of an analytical methodSpike-recovery experiments using SS
End of lessonSignal drift and IS calibration
signal drift
example: increase in signal response to a given sample over time
Signal drift equally affects the detector responses to the analyte and the surrogate standard. Hence, albeit analyte and surrogate standard peak areas change over time, the area ratios remain constant. In contrast to ES calibration, internal standard calibration therefore accounts for signal drift.
