Analyte quantification
Analyte quantification - contextQuantification of analyte
Quantification and standards
External standard (ES) calibration advantage - disadvantage 
Signal drift and ES calib. Matrix effects and ES calib. Analyte loss and ES calib. Internal standard (IS) calibration advantage - disadvantage Signal drift and IS calib. Matrix effects and IS calib. Analyte loss and IS calib. 
Calibration by standard additionWorking range of the detectorNoise of the detectorLimit of detection and quantitationAnalyte recoveryEvaluation of an analytical methodSpike-recovery experiments using SS
End of lessonInternal standard (IS) calibration
... is the most commonly used calibration method in environmental organic trace analysis
... is not susceptible to systematic errors due to signal drift, matrix effects, and analyte loss
... relies on the use of a surrogate standard (SS)
The surrogate standards is a chemical that ...
... exhibits a 'behavior' during sample preparation that is very similar to the 'behavior' of the analyte
... but is, at the same time, analytically distinguishable from the analyte
examples: 
Best internal standard: deuterated analyte However, needs mass selectable detection (mass spectrometry). |
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