Analyte quantification
Analyte quantification - contextQuantification of analyte
Quantification and standards
External standard (ES) calibration advantage - disadvantage 
Signal drift and ES calib. Matrix effects and ES calib. Analyte loss and ES calib. Internal standard (IS) calibration advantage - disadvantage Signal drift and IS calib. 
Matrix effects and IS calib. Analyte loss and IS calib. 
Calibration by standard additionWorking range of the detectorNoise of the detectorLimit of detection and quantitationAnalyte recoveryEvaluation of an analytical methodSpike-recovery experiments using SS
End of lessonMatrix effects and IS calibration
Example: an interferent in the sample (e.g., dissolved organic matter) suppresses the response of the detector to the analyte in the sample relative to the response of the detector to the analyte in a standard, which does not contain the interferent
Matrix components equally suppress the signals of analyte and surrogate standard, such that the ratio of their signal areas remains constant.
Hence, in contrast to ES calibration, internal standard calibration accounts for matrix effects.
