Analyte detection

Analyte detection - context
Detection techniques - purpose
Chromatography - detector system
Detectors ... ?
Coupling chromatography & detectors
     Flame ionization detector (FID)
           General description of FID
           Illustration of FID
           Detailled description of FID
     Electron Capture Detector (ECD)
           General description of ECD
           Illustration of ECD
           Detailled description of ECD
     Comparison FID and ECD
           Type of detector
     UV-visible absorption detector
           General description of UV-vis-AD
                 UV frequency range
                 Principles of light absorption
                       Jablonski scheme
                 Chromophores
           Illustration of the UV-vis-AD
           Detailed description of UV-vis-AD
     Fluorescence detector (FD)
           General description of FD
                 Fluorescent light
           Illustration of FD
           Detailled description of FD
     Mass spectrometric detection (MSD)
           Brief description of MSD
End of lesson


Terminology

Terminology


In the linear dynamic range , the response of a detector is linearly dependent on analyte concentration. Quantification should be performed in this range. Hence, the wider this range, the better.

Limit of linearity (LOL): analyte concentration above which the response of the detector departs from linearity. The higher LOL, the better.

Limit of quantitation (LOQ): lowest possible analyte concentration that can be reliably quantified using a specific analytical method. The lower LOQ, the better.

Limit of detection (LOD): lowest possible analyte concentration that can be reliably detected using a specific analytical method (note: LOD < LOQ). The lower LOD, the better.

Selectivity is defined as the extent to which a detection method can determine a particular analyte without interferences from other chemicals, analytes, or matrix components. The higher, the better for analyte identification.