Analyte detection

Analyte detection - context
Detection techniques - purpose
Chromatography - detector system
Detectors ... ?
Coupling chromatography & detectors
     Flame ionization detector (FID)
           General description of FID
           Illustration of FID
           Detailled description of FID
     Electron Capture Detector (ECD)
           General description of ECD
           Illustration of ECD
           Detailled description of ECD
     Comparison FID and ECD
           Type of detector
     UV-visible absorption detector
           General description of UV-vis-AD
                 UV frequency range
                 Principles of light absorption
                       Jablonski scheme
                 Chromophores
           Illustration of the UV-vis-AD
           Detailed description of UV-vis-AD
     Fluorescence detector (FD)
           General description of FD
                 Fluorescent light
           Illustration of FD
           Detailled description of FD
     Mass spectrometric detection (MSD)
           Brief description of MSD
End of lesson


Terminology

Comparison FID and ECD

 
FID
ECD
 
selectivity
relatively low ; responds to almost all analytes that contain carbon atoms
relatively high ; responds to analytes with groups of high electro-negativity (nitro-, carbonyl-, and halogen groups)

Terminology
limit of quantitation
down to analyte masses of ~ 10-9 g
down to analyte masses of ~ 10-11 g
linear response range
very wide (> 5 orders of magnitude in analyte mass)
narrow (< 3 orders of magnitude in analyte mass)
type of detector
mass flow dependent
concentration dependent