Analyte detection
Analyte detection - context
Detection techniques - purpose
Chromatography - detector system
Detectors ... ?
Coupling chromatography & detectors
Flame ionization detector (FID)
General description of FID
Illustration of FID
Detailled description of FID
Electron Capture Detector (ECD)
General description of ECD
Illustration of ECD
Detailled description of ECD
Comparison FID and ECD
Type of detector
UV-visible absorption detector
General description of UV-vis-AD
UV frequency range
Principles of light absorption
Jablonski scheme
Chromophores
Illustration of the UV-vis-AD
Detailed description of UV-vis-AD
Fluorescence detector (FD)
General description of FD
Fluorescent light
Illustration of FD
Detailled description of FD
Mass spectrometric detection (MSD)
Brief description of MSD
End of lesson
Terminology
Comparison FID and ECD
FID
ECD
selectivity
relatively low ; responds to almost all analytes that contain carbon atoms
relatively high ; responds to analytes with groups of high electro-negativity (nitro-, carbonyl-, and halogen groups)
Terminology
limit of quantitation
down to analyte masses of ~ 10
-9
g
down to analyte masses of ~ 10
-11
g
linear response range
very wide (> 5 orders of magnitude in analyte mass)
narrow (< 3 orders of magnitude in analyte mass)
type of detector
mass flow dependent
concentration dependent