Separation of analytes
Separation of analytes - contextAnalyte mixtures and separation - 1Analyte mixtures and separation - 2Analyte mixtures and separation - 3Introduction to chromatographyChromatographic columnsPrinciple setup of a chromatographic unitExample of separationChromatographic system - 1Chromatographic system - 2Standard gas chromatograph(Old) liquid chromatography unit(New) liquid chromatography unitHPLC columnsComparison GC and HPLC
SelftestProblems
1) GC-columns Answer 2) Which peak corresponds to 1,2,4-TCB? Answer 3) Peak area 100 times larger Answer 4) Unlabeled GC columns Help Answer 5) Which animation is correct? Answer 6) Concept map Hint
End of chapter3) Peak area 100 times larger
Problem:
In the lab you have a standard for 2,4-DNT (crystals with a purity of >98%), which shows 5 peaks of equal areas when injected in its gaseous phase into a GC.
The analytical data sheet for the standard (provided by the company that delivered the standard) also shows 5 peaks with the same column, but the third peak has an area 100 times larger than the other peaks. Obviously, this large peak is the standard. In this analysis the standard was injected not in the gaseous phase but as a solution. What is the explanation for the difference in peak areas between injection in a gaseous phase and injection as a solution?
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