Separation of analytes

Separation of analytes - context
Analyte mixtures and separation - 1
Analyte mixtures and separation - 2
Analyte mixtures and separation - 3
Introduction to chromatography
Chromatographic columns
Principle setup of a chromatographic unit
Example of separation
Chromatographic system - 1
Chromatographic system - 2
Standard gas chromatograph
(Old) liquid chromatography unit
(New) liquid chromatography unit
HPLC columns
Comparison GC and HPLC
Selftest
Problems
End of chapter

Analyte mixtures and separation - 3

 

Instead of extraction with different solvents (or different sorbent phases), use of:

  1. only one solvent (or sorbent phase) as a 'stationary phase'
  2. analytes introduced into a 'mobile phase'
  3. analyte partitioning between mobile and stationary phase

 

 

Slight differences in Ki, stationary phase/mobile phase between analytes result in their separation upon successive re-equilibration between the two phases.

Repeated equilibration of analyte molecules between a stationary phase and a mobile phase is called CHROMATOGRAPHY.