Separation of analytes
Separation of analytes - contextAnalyte mixtures and separation - 1Analyte mixtures and separation - 2Analyte mixtures and separation - 3Introduction to chromatographyChromatographic columnsPrinciple setup of a chromatographic unitExample of separationChromatographic system - 1Chromatographic system - 2Standard gas chromatograph(Old) liquid chromatography unit(New) liquid chromatography unitHPLC columnsComparison GC and HPLC
SelftestProblems
End of chapterChromatographic system - 2
Measuring retention factor Ri:
delay in elution from column relative to a non-sorbing tracer compound
Calculating retention: Transport of analyte only in the mobile phase
e.g. if only 10% are in the mobile phase then R= 1/0.1 = 10
The partitioning constant of analyte between the mobile and the stationary phase may be changed during the course of a chromatographyc run.
Phase Ki, S/M can be altered during a chromatographic run by:
