Analyte detection

Analyte detection - context
Detection techniques - purpose
Chromatography - detector system
     GC sample chromatogram
           GC Run conditions
     HPLC sample chromatogram
           HPLC Run conditions
Detectors ... ?
Coupling chromatography & detectors
End of lesson


Terminology

HPLC sample chromatogram


HPLC Run conditions

 column:  Platinum EPS C18, 5 m, 150 mm x 4.6 mm
 mobile phase:  A: 0.003 M Potassium Phosphate, Monobasic pH 3
 B: Acetonitrile
 gradient (time,%B):  (0, 20), (2, 38), (16, 75)
 flow Rate:  1.4 mL/min
 detector:  UV at 220 nm