Analyte detection
Analyte detection - context
Detection techniques - purpose
Chromatography - detector system
GC sample chromatogram
GC Run conditions
HPLC sample chromatogram
HPLC Run conditions
Detectors ... ?
Coupling chromatography & detectors
End of lesson
Terminology
HPLC sample chromatogram
HPLC Run conditions
column:
Platinum EPS C18, 5
m, 150 mm x 4.6 mm
mobile phase:
A: 0.003 M Potassium Phosphate, Monobasic pH 3
B: Acetonitrile
gradient (time,%B):
(0, 20), (2, 38), (16, 75)
flow Rate:
1.4 mL/min
detector:
UV at 220 nm