Analyte detection

Analyte detection - context
Detection techniques - purpose
Chromatography - detector system
     GC sample chromatogram
           GC Run conditions
     HPLC sample chromatogram
           HPLC Run conditions
Detectors ... ?
Coupling chromatography & detectors
End of lesson


Terminology

GC sample chromatogram


GC Run conditions

 sample:  Environmental soil (1 g); 2 µl (solvent microextraction)
 column:  VB-35, 30m x 0.25mm x 0.5m
 column temp:  120° C for 3 min to 310° C @15° C/min; hold 15 min
 injector temp:  80° C
 detector:  PDD-D3, 300° C
 mobile phase:  helium